Stool Culture

09 Sep.,2024

 

Stool Culture

The stool culture is a test that detects and identifies bacteria that cause infections of the lower digestive tract. The test distinguishes between the types of bacteria that cause disease (pathogenic) and the types that are normally found in the digestive tract (normal flora). The test helps to determine if pathogenic bacteria are the cause of a person&#;s gastrointestinal symptoms (gastroenteritis).

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The bacteria found in stool are representative of the bacteria that are present in the digestive system (gastrointestinal tract). Certain bacteria and fungi called normal flora inhabit everyone&#;s gastrointestinal tract. They play an important role in the digestion of food and their presence keeps a check on the growth of disease-causing bacteria.

Sometimes, the balance of the normal flora may be affected by the administration of broad-spectrum antibiotics; the drugs inhibit the growth of normal flora and allow the bacteria Clostridium difficile that is resistant to the antibiotics to survive and overgrow the digestive tract, leading to symptoms such as diarrhea and abdominal pain.

Pathogenic bacteria can enter and infect the digestive tract when someone eats food or drinks water that is contaminated. Examples of contaminated sources include raw or undercooked eggs, poultry or beef, unpasteurized milk, and untreated water from lakes, streams, and (occasionally) from community water supplies.

People who travel outside the U.S., especially to developing nations, may face a greater risk of being exposed to disease-causing bacteria. Some of these bacteria may be true pathogens while others are strains of gastrointestinal bacteria that are normal flora for the local inhabitants but cause gastrointestinal distress to the tourist. Visitors may become infected by eating or drinking anything that has been contaminated with the bacteria, even things as simple as tap water, ice cubes in a drink, a fresh salad, or food from a vendor&#;s stall.

The most common symptoms of a pathogenic bacterial infection are prolonged diarrhea, bloody diarrhea, mucus in the stool, abdominal pain and cramping, and nausea. If diarrhea lasts more than a few days, it may lead to complications such as dehydration and electrolyte imbalance, which can be dangerous conditions, especially for children and the elderly. Dehydration can cause symptoms such as dry skin, fatigue, and light-headedness.

Severely affected people may require hospitalization to replace lost fluids and electrolytes. Hemolytic uremic syndrome is a serious complication characterized by the destruction of red blood cells and kidney failure that may occasionally arise from an infection with a toxin-producing strain of the bacteria Escherichia coli. The condition is most frequently seen in children, the elderly, and those with weakened immune systems.

If a person&#;s illness is uncomplicated and goes away within a few days, a healthcare practitioner may not order testing. However, if symptoms are severe, if there is bloody diarrhea or mucus present in the stool, or if it is continuing unabated, then a stool culture may be ordered. This is especially true if the person has been outside the U.S. and/or has eaten or drunk anything that has also made someone close to them ill.

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To aid diagnosis, a stool culture may be done in conjunction with or following a GI pathogens panel that simultaneously tests for multiple disease-causing bacteria, viruses, and parasites. Other tests that may be done include an ova and parasite exam or antigen tests to identify specific microbes.

How is the sample collected for testing?

A fresh stool sample is collected in a clean container. The stool sample should not be contaminated with urine or water. Once it has been collected, the stool should be taken to the laboratory within two hours after collection or should be transferred into a vial containing a preservative and taken to the lab as soon as possible. For infants, a stool sample is usually collected with a swab of the rectum.

How to Collect a Stool Sample Using a Stool Collection Kit

This information will teach you how to collect a stool (poop) sample at home using a stool collection kit. Your healthcare provider will talk with you about what they will test your stool sample for. They&#;ll also give you the supplies you need.

You must bring your sample to a lab within 24 hours (1 day) of collecting it.

How to Collect the Sample

  1. Wash your hands. Wet your hands and apply soap. Rub your hands together well for at least 20 seconds, then rinse. Dry your hands with a paper towel and use that same towel to turn off the faucet.
  2. Gather your supplies. You&#;ll need:
  • Collection hat
  • Wooden stick
  • Biohazard bag
  • Prelabeled specimen cup(s)

Place them in the bathroom where you can reach them easily.

  1. Lift the toilet seat. Place the collection hat on the toilet bowl rim. Close the toilet seat on the collection hat. This will hold the collection hat in place.
  2. Sit on the toilet to have a bowel movement (poop) as usual. Don&#;t pee on your stool or in the collection hat.
  3. Using the wooden stick, place 2 to 3 small scoopfuls of stool into each specimen cup(s).
  4. Place the specimen cup(s) inside the plastic biohazard bag.
  5. Throw away the collection hat and wooden stick.
  6. Wash your hands again.

After You Collect the Sample

Store the biohazard bag with your stool sample at room temperature. Bring it to a lab within 24 hours of collecting the sample.

Your healthcare provider will tell you where to drop off the sample and when the lab is open. It&#;s best to bring it to a Memorial Sloan Kettering (MSK) lab, if you can. If you want to bring your sample to a lab closer to your home, talk with your healthcare provider.

Contact your healthcare provider if you have any questions or concerns.